Molecular Delivery into Live Cells with Light Image Patterns and a Gold Particle Coated Substrate
نویسندگان
چکیده
We present a method for permeabilizing cells using a disposable substrate coated with gold nanoparticles. Upon laser irradiation, the unique photothermal properties of the gold nanoparticles facilitate selective permeabilization of the cellular membrane using patterned laser images. The permeabilization of the cell membrane is characterized by intercalation of fluorescent Calcein dye.
منابع مشابه
Image patterned molecular delivery into live cells using gold particle coated substrates
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as...
متن کاملBiolistic co-transformation of rice using gold nanoparticles
ABSTRACT- In order to produce transgenic rice lines lacking selectable marker gene, biolistic co-transformation technique using gold nanoparticles was adopted. In the first step, the efficiency of different sizes of gold particles was evaluated. The results showed that the efficiency of the nanoparticles in the transformation was comparable to that of the micro particles. Subsequently, two sepa...
متن کاملEffect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells
Background: Cell penetrating peptides (CPPs) can enter a cell through the cell membrane, and used in the fields of drug delivery, gene therapy, and cancer therapy by their property transporting various molecules into cytoplasm. Gold nanospheres (GNSs) are a useful tool for molecular imaging, because they are not cytotoxic and have high solubility, excellent light scattering property and ease of...
متن کاملInvestigation of acoustic properties of silica coated gold nanoparticle as contrast agent for Ultrasonography
Interoduction: Ultrasound images have often low contrast due to small differences in acoustic impedance between different tissues. Air or gas microbubbles that surrounded by membrane are most of the contrast agents in ultrasound imaging. Problems such as instability in sound pressure and inability in penetrating from the blood vessel into body tissues limited the use of microbubbles into the in...
متن کاملEntrapped chemically synthesized gold nanoparticles combined with polyethylene glycol and chloroquine diphosphate as an improved antimalarial drug
Objective(s): Drug delivery is an engineering technology to control the release and delivery of therapeutic agents to target organs, tissues, and cells. Metallic nanoparticles, such as gold nanoparticles (AuNPs) have exceptional properties which enable efficient drug transport into different cell types with reduced side effects and cytotoxicity to other tissues.Materials and Methods: AuNPs were...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008